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みなさん、こんにちは。渡邉力也です。2人の子供の父親であるとともに、好奇心の赴くままに、20年近くバイオと工学の研究活動を続けてきました。毎日、色々な発見と出会いがあり、この世界のすばらしさを実感しています。今後は、仲間と更なる人生の航海にでるとともに、科学技術を通じて、より良い社会の実現に貢献できるようがんばっていきたいと思います。

Let's enjoy science and engineering for a better society!

Curriculum Vitae

1981年　静岡県沼津市生まれ

これまで住んだ町
沼津市、杉並区、文京区、豊島区、吹田市、箕面市

学歴
2000年　静岡県立沼津東高等学校理数科 卒業
2004年　早稲田大学理工学部機械工学科 卒業
2006年　東京大学大学院工学系研究科 修士課程 修了
2009年　大阪大学大学院工学研究科 博士課程 修了

職歴
2009年　大阪大学産業科学研究所 特任研究員
2011年　東京大学大学院工学系研究科応用化学専攻 助教
2013年　科学技術振興機構 さきがけ研究員 (兼任)
2016年　東京大学大学院工学系研究科応用化学専攻 講師
2018年　理化学研究所開拓研究本部 主任研究員
2020年　分子科学研究所 客員教授 (兼任)
2022年　東京医科歯科大学 連携教授 (兼任)
2024年　埼玉大学 連携教授 (兼任)

Publications

    2024
    Iida, T., Ando, J., Yoshimura, M., Makino, A., Nakano, M., Kogo, Y., Shinoda, H., Toyoda, M., Noda, T., & *Watanabe, R.
    “Portable wide-field femtoliter-chamber imaging system for point-of-care digital bioanalysis”
    iScience (2024) 110868

    *Ando, J., Murai, K., Mori, M., Michiyuki, T., Iida, T., Makino, A., Shinoda, H., & *Watanabe, R.
    “Exploring fluoropolymers for fabrication of femtoliter chamber arrays used in digital bioanalysis”
    Sci Rep (2024) 14, 11442

    Sakamoto, S., Hiraide, H., Minoda, M., Iwakura, N., Suzuki, M., Ando, J., Takahashi, C., Takahashi, I., Murai, K., Kagami, Y., Mizuno, T., Koike, T., Nara, S., Morizane, C., Hijioka, S., Kashiro, A., Honda, K., Watanabe, R., *Urano, Y & *Komatsu, T.
    “Identification of activity-based biomarkers for early-stage pancreatic tumors in blood using single-molecule enzyme activity screening”
    Cell Rep Methods (2024) 100688


    2023
    Ukegawa, T., *Komatsu, T., Minoda, M., Matsumoto, T., Iwasaka, T., Mizuno, T., Tachibana, R., Sakamoto, S., Hanaoka, K., Kusuhara, H., Honda, K., Watanabe, R., & *Urano, Y.
    “Thioester-Based Coupled Fluorogenic Assays in Microdevice for the Detection of Single-Molecule Enzyme Activities of Esterases with Specified Substrate Recognition”
    Adv Sci (2023) e2306559

    Ando, J., & *Watanabe, R.
    “Toward versatile digital bioanalysis”
    Biomicrofluidics (2023) 17, 061303

    Iida, T., Shinoda, H., & *Watanabe, R.
    “SATORI: Amplification-free digital RNA detection method for the diagnosis of viral infections”
    Biophys Physicobiol (2023) e200031

    Ueda, T., *Shinoda, H., Makino, A., Yoshimura, M., Iida, T., & *Watanabe, R.
    “Purification/Amplification-Free RNA Detection Platform for Rapid and Multiplex Diagnosis of Plant Viral Infections”
    Anal Chem (2023) 95, 9680-9686

    Kastian, RF., Baba, K., Kaewkascholkul, N., Sasaki, H., Watanabe, R., Toriyama, M., & *Inagaki, N.
    “Dephosphorylation of neural wiring protein shootin1 by PP1 phosphatase regulates netrin-1-induced axon guidance”
    J Biol Chem (2023) 299, 1046687

    Nagano, N., Ichihashi, Y., Komatsu, T., Matsuzaki, H., Hata, K., Watanabe, T., Misawa, Y., Suzuki, M., Sakamoto, S., Kagami, Y., Kashiro, A., Takeuchi, K., Kanemitsu, Y., Ochiai, H., Watanabe, R., Honda, K., & *Urano, Y.
    “Development of fluorogenic substrates for colorectal tumor-related neuropeptidases for activity-based diagnosis”
    Chem Sci (2023) 14, 4495-4499

    Iida, T., Ando, J., Shinoda, H., Makino, A., Yoshimura, M., Murai, K., Mori, M., Takaeuchi, H., Noda, T., Nishimasu, H., & *Watanabe, R.
    “Compact wide-field femtoliter-chamber imaging system for high-speed and accurate digital bioanalysis”
    Lab Chip (2023) 23, 684-691


    2022
    Shinoda, H., Iida, T., Makino, A., Yoshimura, M., Ishikawa, J., Ando, J., Murai, K., Sugiyama, K., Muramoto, Y., Nakano, M., Kiga, K., Cui, L., Nureki, O., Takaeuchi, H., Noda, T., *Nishimasu, H., & *Watanabe, R.
    “Automated amplification-free digital RNA detection platform for rapid and sensitive SARS-CoV-2 diagnosis”
    Commun Biol (2022) 5, 473


    2021
    Shinoda, H., Taguchi, Y., Nakagawa, R., Makino, A., Okazaki, S., Nakano, M., Muramoto, Y., Takahashi, C., Takahashi, I., Ando, J., Noda, T., *Nureki, O., *Nishimasu, H., & *Watanabe, R.
    “Amplification-free RNA detection with CRISPR-Cas13”
    Commun Biol (2021) 4, 476


    2020
    Soga, N., Ota, A., Nakajima, K., Watanabe, R., Ueno, H., & *Noji, H.
    “Monodisperse Liposomes with Femtoliter Volume Enable Quantitative Digital Bioassays of Membrane Transporters and Cell-Free Gene Expression”
    ACS Nano (2020) 14, 11700-11711

    Sakamoto, S., *Komatsu, T., *Watanabe, R., Zhang, Y., Inoue, T., Kawaguchi, M., Nakagawa, H., Ueno, T., Okusaka, T., Honda, K., *Noji, H., & *Urano, Y.
    “Multiplexed single-molecule enzyme activity analysis for counting disease-related proteins inbiological samples”
    Sci Adv (2020) 6, eaay0888


    2018
    *Watanabe, R., Komatsu, T., Sakamoto, S., Urano, Y., & *Noji, H.
    “High-throughput single-molecule bioassay using micro-reactor arrays with a concentration gradient of target molecules”
    Lab Chip (2018) 18, 2849-2853

    Baba, K., Yoshida, W., Toriyama, M., Shimada, T., Manning, C., Saito, M., Kohno, K., Trimmer, J., Watanabe, R., & *Inagaki, N.
    “Gradient-reading and mechano-effector machinery for netrin-1–induced axon guidance”
    eLife (2018) 7, e34593

    Moriizumi, Y., *Tabata, KV, Watanabe, R., Doura, T., Kamiya, M., Urano, Y., & *Noji, H.
    “Hybrid cell reactor system from Escherichia coli protoplast cells and arrayed lipid bilayer chamber device”
    Sci Rep (2018) 8, 11757

    *Watanabe, R., Sakuragi, T., *Noji, H., & *Nagata, S.
    “Single molecule analysis of phospholipid scrambling by TMEM16F”
    Proc Natl Acad Sci USA (2018b) 115, 3066-3071

    Abe, K., Katsuno, H., Toriyama, M., Baba, K., Mori, T., Hakoshima, T., Kanemura, Y., Watanabe, R., & *Inagaki, N.
    “Grip and slip of L1-CAM on adhesive substrates direct growth cone haptotaxis”
    Proc Natl Acad Sci USA (2018a) 115, 2764-2769

    *Watanabe, R., Soga, N., Ohdate, S., & *Noji, H.
    “Single molecule analysis of membrane transporter activity by using a microsystem”
    Methods Mol Biol (2018) 1700, 321-330

    *Watanabe, R., Genda, M., Kato-Yamada, Y., & *Noji, H.
    “Essential role of the epsilon subunit for reversible chemo-mechanical coupling in F1-ATPase”
    Biophys J (2018) 114, 178-187

    Tamiya, Y., Watanabe, R., Noji, H., *Li, C., & *Komatsuzaki, T.
    “Effects of non-equilibrium angle fluctuation on F1-ATPase kinetics induced by temperature increase”
    Phys Chem Chem Phys (2018) 20, 1872-1880


    2016
    *Watanabe, R., Soga, N., Hara, M., & *Noji, H.
    “Arrayed water-in-oil droplet bilayers for membrane transport analysis”
    Lab Chip (2016) 16, 3043-3048

    *Watanabe, R., Soga, N., & *Noji, H.
    “Novel nano-device to measure voltage-driven membrane transporter activity”
    IEEE Trans Nanotechnol (2016) 15, 70-73

    *McMiillan, D. G., Watanabe, R., Ueno, H., Cook, G. M., & *Noji, H.
    “Biophysical Characterization of a Thermoalkaliphilic Molecular Motor with a High Stepping-Torque Gives Insight into Evolutionary ATP Synthase Adaptation”
    J Biol Chem (2016) 291, 23965-23977


    2015
    Li, C. B., Ueno, H., Watanabe, R., Noji, H., & *Komatsuzaki, T.
    “ATP hydrolysis assists Phosphate release and promotes reaction ordering in F1-ATPase”
    Nat Commun (2015) 6, 10223

    Soga, N., *Watanabe, R., & *Noji, H.
    “Attolitre-sized lipid bilayer chamber array for rapid detection of single transporters”
    Sci Rep (2015) 5, 11025

    Watanabe, R., Koyasu, K., You, H., Tanigawara, M., & *Noji, H.
    “Torque transmission mechanism via DELSEED loop of F1-ATPase”
    Biophys J (2015) 108, 1144-52

    Yukawa, A., *Watanabe, R., & *Noji, H.
    “Effects of an ATP analogue, adenosine 5'-[α-thio]triphosphate, on F1-ATPase rotary catalysis, torque generation, and inhibited intermediated formation”
    Biochem Biophys Res Commun (2015) 458, 515-9

    Yukawa, A., Iino, R., Watanabe, R., Hayashi, H., & *Noji, H.
    “Key Chemical Factors of Arginine Finger Catalysis of F1-ATPase Clarified by an Unnatural Amino Acid Mutation”
    Biochemistry (2015) 54, 472-80


    2014
    *Watanabe, R., Soga, N., Yamanaka, T., & *Noji, H.
    “High-throughput formation of lipid bilayer membrane arrays with an asymmetric lipid composition”
    Sci Rep (2014) 4, 7076

    Watanabe, R., Minagawa, Y., & *Noji, H.
    “Thermodynamic analysis of F1-ATPase rotary catalysis using high-speed imaging”
    Protein Sci (2014) 23, 1773-1779

    *Watanabe, R., Soga, N., Fujita, D., Tabata, K. V., Yamauchi, L., Kim, SH., Asanuma, D., Kamiya, M., Urano, Y., *Suga, H., & *Noji, H.
    “Arrayed lipid bilayer chambers allow single-molecule analysis of membrane transporter activity”
    Nat Commun (2014b) 5, 4519

    Watanabe, R., Matsukage, Y., Yukawa, A., Tabata, K. V., & *Noji, H.
    “Robustness of the Rotary Catalysis Mechanism of F1-ATPase”
    J Biol Chem (2014) 289, 19331-19340

    Arai, HC., Yukawa, A., Iwatate, RJ., Kamiya, M., Watanabe, R., Urano, Y., & *Noji, H.
    “Torque generation mechanism of F1-ATPase upon NTP binding”
    Biophys J (2014) 107, 156-64

    Watanabe, R., & *Noji, H.
    “Characterization of the temperature-sensitive reaction of F1-ATPase by using single-molecule manipulation”
    Sci Rep (2014) 4, 4962

    Watanabe, R., & *Noji, H.
    “Timing of inorganic phosphate release modulates the catalytic activity of ATP-driven rotary motor protein”
    Nat Commun (2014a) 5, 3486


    2013
    Watanabe, R., Hayashi, K., Ueno, H., & *Noji, H.
    “Catalysis-enhancement via rotary fluctuation of F1-ATPase”
    Biophys J (2013) 105, 2385-91

    Watanabe, R., Tabata, KV., Iino, R., Ueno, H., Iwamoto, M., Oiki, S., & *Noji, H.
    “Biased Brownian stepping rotation of FoF1-ATP synthase driven by proton motive force”
    Nat Commun (2013) 4, 1631


    2012
    Tanigawara, M., Tabata, KV., Ito, Y., Ito, J., Watanabe, R., Ueno, H., Ikeguchi, M., & *Noji, H.
    “Role of the DELSEED Loop in Torque Transmission of F1-ATPase”
    Biophys J (2012) 103, 970-8

    You, H., Iino, R., Watanabe, R., & *Noji, H.
    “Winding single-molecule double-stranded DNA on a nanometer-sized reel”
    Nucleic Acids Res (2012) 40, e151

    Watanabe, R., Okuno, D. Sakakihara, S. Shimabukuro, K. Iino, R. Yoshida, M. & *Noji, H.
    “Mechanical modulation of catalytic power on F1-ATPase”
    Nat Chem Biol (2012) 1, 86-92


    2010
    Watanabe, R., Iino, R. & *Noji, H.
    “Phosphate release in F1-ATPase catalytic cycle follows ADP release.”
    Nat Chem Biol (2010) 6, 814-20


    2009
    Enoki, S., Watanabe, R., Iino, R. & *Noji, H.
    “Single-molecule study on the temperature-sensitive reaction of F1-ATPase with a hybrid F1 carrying a single beta(E190D).”
    J Biol Chem (2009) 284, 23169-76


    2008
    Watanabe, R., Iino, R., Shimabukuro, K., Yoshida, M. & *Noji, H.
    “Temperature-sensitive reaction intermediate of F1-ATPase.”
    EMBO Rep (2008) 9, 84-90
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